## Abstract Using the enzymeβlinked immunosorbent assay (ELISA), we detected antibodies to interstitial (type I) and basement membrane (type IV) collagens in the sera of patients with scleroderma (systemic sclerosis). Antibodies against type IV collagen were found in significant levels in these pat
Antibodies to collagen in scleroderma
β Scribed by M. J. Rowley; L. J. McNeilage; I. R. Mackay
- Book ID
- 111698659
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 131 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0004-3591
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Peripheral blood mononuclear cells (PBMC) obtained from scleroderma patients and normal controls were cultured in the presence of types I and IV collagen, intima collagen, laminin, and phytohemagglutinin. PBMC from 9 of 19 patients (47%) demonstrated lymphocyte transformation in response to laminin,
When HeLa cells were used as the substrate for detection by the indirect immunofluorescence method, antinuclear antibodies were demonstrated in 16 of 22 (72.7%) sera from patients with localized scleroderma. When mouse kidney sections were used, the positive rate for antinuclear antibodies was 50% (