Antibodies to 4-hydroxyandrostenedione — a new anti-breast cancer agent suitable for use in a radioimmunoassay

✍ Scribed by J. Khubieh; G. W. Aherne; J. Chakraborty

• Publisher: Springer
• Year: 1987
• Tongue: English
• Weight: 160 KB
• Volume: 19
• Category: Article
• ISSN: 0344-5704
• DOI: 10.1007/bf00254575

✦ Synopsis

A specific antiserum suitable for radioimmunoassay (RIA) of 4-hydroxy-4-androstene-3,17 dione (4-OHA) has been developed. Sheep antiserum was raised by injecting two different conjugates prepared by coupling 4-OHA to ovalbumin. Antisera obtained from a sheep immunised with 4-hydroxy-testosterone-17-hemisuccinate ovalbumin conjugate were of higher titre and more specific than antisera obtained from sheep immunised with 4-hydroxyandrostenedione-7 alpha-carboxyethylthioether. The antiserum bound 50% of 20 picograms of [6,7-3H]-4-OHA at an initial dilution of 1:270. The most relevant steroids, androstenedione (AD) and testosterone (T) were tested and showed cross reactivity of 2.7% and 5.1% respectively. The lower limit of detection was 4.5 pg/tube. Antisera from this animal will prove useful as the basis of a sensitive and specific RIA for clinical pharmacokinetic studies of 4-OHA.