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Antibodies against Double-Stranded DNA in Patients with Connective Tissue Diseases

✍ Scribed by Permin, Henrik ;Halberg, Poul ;Christiansen, Erik

Book ID
114691471
Publisher
Wiley (Blackwell Publishing)
Year
2009
Weight
348 KB
Volume
203
Category
Article
ISSN
0001-6101

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A simple and sensitive enzyme-linked immunosorbent assay (ELISA) is described for the measurement of antibodies to native DNA (double-stranded) and to heat-denatured DNA (single-stranded) in sera of patients with connective tissue disorders. DNA bound to polyvinylchloride plates is incubated with se