Antibiotic uptake by bacteria as measured by partition in polymer aqueous phase systems

✍ Scribed by Nicole Moreau; Patricia Lacroix; Laurent Fournel

• Book ID: 102628461
• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 490 KB
• Volume: 141
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(84)90430-5

✦ Synopsis

Polyethyleneglycol/dextran and polyethyleneglycol/salt two-phase systems are used to measure the entry of antibiotics into Escherichia coli and Staphylococcus aureus cells. Aminoglycosides, macrolides, a quinolone, and a cycline were assayed. The method is simple and rapid and eliminates the problems encountered with filters, especially nonspecific binding; it allows rapid uptake kinetics to be measured.