Anti-β4 integrin antibodies enhance migratory and invasive abilities of human colon adenocarcinoma cells and their MMP-2 expression
✍ Scribed by Noucha Daemi; Nicole Thomasset; Jean-Claude Lissitzky; Jérôme Dumortier; Marie-France Jacquier; Céline Pourreyron; Patricia Rousselle; Jean-Alain Chayvialle; Lionel Remy
- Book ID
- 101234908
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- French
- Weight
- 300 KB
- Volume
- 85
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Integrin-mediated adhesion of cells to extracellular matrix proteins has been shown to activate various intracellular signaling events. In the present study, we demonstrate that the addition of a monoclonal antibody raised against the 4 integrin subunit in the culture medium of a clone derived from the colon adenocarcinoma cell line LoVo specifically results in stimulation of cell migration and invasion through reconstituted basement membrane matrices. Moreover, an increase in MMP-2 activity is observed. Conversely, monoclonal anti-␣6 and anti-1 have no effect on MMP-2 expression. The s.c. co-injection of adenocarcinoma cells with antibodies raised against the 4 integrin subunit to immunosuppressed newborn rats gives rise to tumors displaying altered and disorganized peri-tumoral basement membranes compared with tumors obtained when cells are injected with adenocarcinoma cells alone. Higher metastatic capacity of cells results when they are co-injected with antibodies to the 4 integrin subunit. Our results suggest that the 4 subunit of ␣64 integrin, a laminin receptor in colon adenocarcinoma, may be responsible for the specific signals which stimulate cell motility, expression of MMP-2 and tumor invasion. Int.