Anti-norovirus polyclonal antibody and its potential for development of an antigen-ELISA

✍ Scribed by Michio Okame; Tomoyuki Shiota; Grant Hansman; Makiko Takagi; Fumihiro Yagyu; Sayaka Takanashi; Tung Gia Phan; Yuko Shimizu; Hideki Kohno; Shoko Okitsu; Hiroshi Ushijima

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 136 KB
• Volume: 79
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.20906

✦ Synopsis

Abstract

Norovirus (NoV) capsid proteins were expressed as virus‐like particles (VLPs) by using recombinant baculovirus in insect cells, which had 5 genotypes in genogroup I and 11 genotypes in genogroup II, and the VLPs were used as immunogens. Polyclonal antibody against the VLP of GII/3 genotype showed broad‐range cross‐reactivity, reacting not only with intra‐genogroup strains, but also inter‐genogroup strains, by antibody‐ELISA using 16 kinds of VLPs. Furthermore, antigen‐ELISA was conducted in sandwich enzyme‐linked immunosorbent assay (ELISA) using the polyclonal antibody for capturing antigens, and three kinds of monoclonal antibodies against the VLP of GII/4 genotype for detecting antigens. This format successfully detected eight genotypes of NoV from clinical specimens and proved that polyclonal antibody, which has broad‐range cross‐reactivity, was capable of detecting various types of genotypes from clinical specimens. J. Med. Virol. 79: 1180–1186, 2007. © 2007 Wiley‐Liss, Inc.