Anti-lymphocytic antibodies and marrow transplantation. III. Effect of heterologous anti-brain antibodies on acute secondary disease in mice

✍ Scribed by H. Rodt; S. Thierfelder; M. Eulitz

• Publisher: John Wiley and Sons
• Year: 1974
• Tongue: English
• Weight: 528 KB
• Volume: 4
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830040107

✦ Synopsis

Abstract

Antisera against murine thymus‐derived lymphocytes were raised in rabbits injected with mouse brain. It was possible to remove contaminating antibodies which cross‐reacted with B cells and hemopoietic stem cells by absorption with liver and plasmacytoma followed by purification and fractionation over DEAE‐cellulose. T cell specificity of the absorbed anti‐mouse brain serum (AMBS) and its purified globulin fraction (AMBG) was demonstrated by cytotoxicity, complement fixation and unaltered numbers of plaque‐forming cells and colony‐forming units. In vivo, the effect of absorbed AMBG on the graft‐versus‐host reaction was investigated in an H‐2‐incompatible parent‐to‐F~1~ hybrid donor‐recipient combination. (C57BL/6 × CBA)F~1~ mice irradiated with 900 rad and transfused with C57BL/6 spleen cells died from acute secondary disease within 20 days. They survived as complete chimaeras beyond day 300 without symptoms of secondary disease when the donor cells had been preincubated with absorbed AMBG. The implications of the suppression of secondary disease in this model are discussed.