Anti-inflammatory function of Withangulatin A by targeted inhibiting COX-2 expression via MAPK and NF-κB pathways

✍ Scribed by Lijuan Sun; Jianwen Liu; Daling Cui; Jiyu Li; Youjun Yu; Lei Ma; Lihong Hu

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 328 KB
• Volume: 109
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.22430

✦ Synopsis

Withangulatin A (WA), an active component isolated from Physalis angulata L., has been reported to possess anti-tumor and trypanocidal activities in model systems via multiple biochemical mechanisms. The aim of this study is to investigate its anti-inflammatory potential and the possible underlying mechanisms. In the current study, WA significantly suppressed mice T lymphocytes proliferation stimulated with LPS in a dose-and time-dependent manner and inhibited pro-inflammation cytokines (IL-2, IFN-g, and IL-6) dramatically. Moreover, WA targeted inhibited COX-2 expression mediated by MAPKs and NF-kB nuclear translocation pathways in mice T lymphocytes, and this result was further confirmed by the COX-1/2 luciferase reporter assay. Intriguingly, administration of WA inhibited the extent of mice ear swelling and decreased pro-inflammatory cytokines production in mice blood serum. Based on these evidences, WA influences the mice T lymphocytes function through targeted inhibiting COX-2 expression via MAPKs and NF-kB nuclear translocation signaling pathways, and this would make WA a strong candidate for further study as an anti-inflammatory agent.