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Anti-idiotypic antibodies to anti–PDC-E2 in primary biliary cirrhosis and normal subjects

✍ Scribed by Qiao-yi Chen; Merrill J. Rowley; Ian R. Mackay

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
120 KB
Volume
29
Category
Article
ISSN
0270-9139

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✦ Synopsis

Anti-idiotypic antibodies may regulate the immune system and influence pathogenic autoimmunity. We investigated idiotype-anti-idiotype interactions in sera of patients with primary biliary cirrhosis (PBC), normal subjects and animals immunized with pyruvate dehydrogenase complex (PDC) or its derivatives. IgG autoantibody to the E2 subunit of PDC (PDC-E2) was derived by affinity-purification from sera of 12 patients with PBC, and F(ab) 2 was prepared (anti-PDC-E2-F[ab] 2 ). This was used as a reactant by enzyme-linked immunosorbent assay (ELISA) with sera from patients with PBC, normal subjects, or immunized animals. Results were that IgG antibody to anti-PDC-E2-F(ab) 2 was detectable at low concentration in 12 PBC sera (mean optical density [OD] ؎ SD: 1.02 ؎ 0.26), and also in 19 normal sera (mean OD ؎ SD: 0.97 ؎ 0.35) using a serum dilution of 1:20; background OD was 0.09 to 0.10, whereas antisera from animals immunized with PDC or PDC-E2 were nonreactive. There was a significant inverse correlation (r ‫؍‬ ؊.

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