Anti-DNA.RNA sera

✍ Scribed by A. K. Raap; J. G. J. Marijnen; M. Ploeg

• Book ID: 104785946
• Publisher: Springer
• Year: 1984
• Tongue: English
• Weight: 470 KB
• Volume: 81
• Category: Article
• ISSN: 1432-119X
• DOI: 10.1007/bf00489529

✦ Synopsis

The specificity of anti-nucleic acid antisera can immunocytochemically be evaluated with test systems which apply various nucleic acids immobilized to inert matrices. When using polyrA.polyrU as a model compound for dsRNA, it is important to prevent the formation of the triple stranded form polyrA. (polyrU)a.

Anti-dsRNA antibodies which, when tested with the correct test system, proved to be present in an earlier described anti-DNA.RNA serum, could be removed by adsorption. By cytofluorometric comparison of the immunofluorescence signals obtained with the anti-dsRNA containing serum and the absorbed serum, it could be shown that the anti-dsRNA antibodies do not contribute to the specific signals measured after in situ hybridization.

Repetitive incubations with the anti-DNA.RNA serum led to a considerable increase in immunofluorescence signal. * To whom reprint requests should be sent This work was subsidized in part by Het Praeventiefonds, The Hague, The Netherlands considerable increase in immunofluorescence signals without an important increase of the background.