Anti-120-kDa α-fodrin immune response with Th1-cytokine profile in the NOD mouse model of Sjögren's syndrome
✍ Scribed by Kumiko Yanagi; Naozumi Ishimaru; Norio Haneji; Kaoru Saegusa; Ichiro Saito; Yoshio Hayashi
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 174 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
Our recent study suggested that the 120-kDa § -fodrin molecule may be an important autoantigen in the pathogenesis of Sjögren's syndrome, and anti-120-kDa § -fodrin antibodies have been detected in patients with Sjögren's syndrome. Here we have analyzed anti-120-kDa § -fodrin immune responses during development of spontaneous autoimmune sialadenitis in NOD mice as a model of Sjögren's syndrome. We found specific autoantibody production against 120-kDa § -fodrin, and its production correlated closely with autoimmune sialadenitis. A specific T cell response of splenocytes against the 120-kDa § -fodrin autoantigen was observed in NOD mice from the early onset of autoimmune sialadenitis. In addition, production in vitro by splenic T cells of cytokines such as interleukin-2 (IL-2) and interferon-+ (IFN-+ ), but not IL-4, was detected by enzyme-linked immunosorbent assays. We found upregulation of local cytokine genes, including those of Th1 type (IL-1 g , TNF- § , IL-2, IFN-+ , IL-6), as well as IL-10 and IL-12(p40), in the tissue-infiltrating cells during the course of autoimmune sialadenitis. These findings suggest that in spontaneous autoimmune sialadenitis in NOD mice, there may be a specific anti-120-kDa § -fodrin immune response in the development of autoimmune lesions resembling human Sjögren's syndrome, and that the autoreactive Th1 cells possess an up-regulated cytokine profile besides IL-10 and IL-12.