𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Anther cultures ofNicotiana tabacumL. mutants

✍ Scribed by J. Vagera; F. J. Novák; B. Vyskot

Book ID
104698012
Publisher
Springer
Year
1976
Tongue
English
Weight
499 KB
Volume
47
Category
Article
ISSN
0040-5752

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✦ Synopsis

The theoretically expected and experimentally observed phenotypic ratios have been compared in populations of haploids derived from chlorophyll mutants of Nicotiana tabacum L. with a known genotypic constitution. The frequencies of mutant genotypes were significantly lower than the expected values, proving the existence of selection in a system of haploid embryoids developing in the anther.The anthers from M1 plants of a diploidized Nicotiana tabacum haploid cv. Samsun, treated with various concentrations of N-nitroso-N-methylurea and n-butylmethane sulphonate, were cultivated in vitro. The number of anthers which gave rise to haploids (embryogenic anthers) was stimulated by lower concentrations of both the mutagens. The stimulation at the level of M1 sporophyte is explained by internal genetic heterogeneity induced by adequate mutagen concentration. The average number of haploids per embryogenic anther decreased in all the treatments. The frequency of haploid plants of the mutant phenotypes increased with increasing mutagen concentration.

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