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Anomalously high cooperativity of oligodeoxycytidylic acid for luminescence resonance energy transfer to lanthanide ions

✍ Scribed by Jihee Lee; Minyoung Park; Hyung Su Son; Sang Bum Lee; Hee Cheon Lee; Ja Kang Ku; Joon Won Park


Publisher
Wiley (John Wiley & Sons)
Year
2002
Tongue
English
Weight
248 KB
Volume
67
Category
Article
ISSN
0006-3525

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✦ Synopsis


The luminescence of terbium(III) and europium(III) through luminescence resonance energy transfer from mononucleotides and oligodeoxynucleotides is examined. Among mononucleotides, dGMP gives the strongest luminescence of terbium(III), while dTMP and dCMP yield a luminescence intensity of europium(III) that is larger than the other two cases. In the homodeoxydecamers, decadeoxycytidylic acid (dC10) produces the highest intensity for both metals. The anomalously large cooperativity of dC10 is explained by the easiness of deformation of the helical structure to bind lanthanide ions, and a circular dichroism study supports this explanation.