Anomalous tritium loss in the measurement of tissue hydroxy-[5-3H]proline specific activity following chloramine-T oxidation
✍ Scribed by Geoffrey J. Laurent; Robin J. McAnulty; Michael H. Oliver
- Book ID
- 102985846
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 568 KB
- Volume
- 123
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Studies of collagen metabolism are most often accomplished by administering labeled proline to animals with subsequent measurement of the hydroxyproline specific activity. The most commonly employed method for making such measurements is based on the chloramine-T oxidation of hydroxyproline to form pyrrole (Prockop and Udenfriend, (1960), Anal. Biochem. 1, 228). When these methods were used to measure hydroxyproline specific activities in tissues of animals given [S-'Hlproline and [U-"'Clproline, it was observed that the tritium loss in the reaction, for which correction is normally made, was not as predicted. This means a correction factor different from that conventionally employed should be used in calculating the specific activity of hydroxyproline.