Anomalous interaction of 2-thiopyridone with proteins during thiol-disulfide interchange reactions

✍ Scribed by Robert B. Harris; Leonard T. Hodgins

• Publisher: Elsevier Science
• Year: 1980
• Tongue: English
• Weight: 215 KB
• Volume: 109
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(80)90643-0

✦ Synopsis

Unpredictable results were observed for the formation of 2-thiopyridone during the reaction of protein-thiol groups with 2,2'-dipyridyl disulfide. Spectrophotometric analyses revealed that 2-thiopyridone associated noncovalently with reduced or unreduced proteins to cause a hypsochromic shift from 343 to 302 nm. Such complex formation compromises quantification of the thiol-disulfide exchange reaction.