Anomalies in sedimentation. III. A model for the inherent instability of solutions of very large particles in high centrifugal fields
✍ Scribed by Verne N. Schumaker; Bruno H. Zimm
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1973
- Tongue
- English
- Weight
- 915 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0006-3525
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✦ Synopsis
Abstract
Sedimentation, diffusion, and hydrodynamic interaction are included in a simplified model for the behavior of a solution macromolecules in a centrifugal field. The model is simulated by a program on a digital computer. As the diffusion coefficient is allowed to decrease, the simulation shows that the molecules tend to cluster in high centrifugal fields. The clusters move through the solution in the direction of sedimentation in a wave‐like fashion, acquiring new molecules at the front and shedding old ones to the rear. It is suggested that this tendency to cluster could account for the loss of DNA and other very large molecules from solution which is observed to occur at high centrifugal fields.