Annual Review of Cell Biology, Volume 9, 1993 (GEORG E. PALADE, BRUCE M. ALBERTS and JAMES A. SPUDICH, Editors) XII + 659 S., 58 Abb., 21 Tab. Palo Alto 1993. Annual Reviews Inc. $ 51.00, ISBN: 0–8243–3109–5

✍ Scribed by K. J. Böhme

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 81 KB
• Volume: 35
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.3620350104

✦ Synopsis

Schizosaccharomyces pombe is becoming a more and more important experimental organism. This manual, written after several Cold Spring Harbor courses "Molecular Genetics of Fission Yeast" is helpful to introduce newcomers to the basic principles of fission yeast cell biology, molecular biology, and genetics. It provides a guide to mutant isolation, chromosome mapping, gene cloning and disruption, cell fractionation and nuclear isolation. It also introduces the principles of cell synchronisation, flow cytometry, and immunofluorescence microscopy.

The book has three sections, including 2 1 experimental protocols, twelve appendics, a list of suppliers and references.

The manual can be addressed to students, post-graduates and all investigators starting experiments with fission yeast.

C. KURISCHKO (Jena)