Annexin expressions are temporally and spatially regulated during rat hepatocyte differentiation

✍ Scribed by Bruno Della Gaspera; Françoise Braut-Boucher; Morgane Bomsel; Françoise Chatelet; Christiane Guguen-Guillouzo; Jacqueline Font; Jacqueline Weinman; Serge Weinman

• Book ID: 102818470
• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 513 KB
• Volume: 222
• Category: Article
• ISSN: 1058-8388
• DOI: 10.1002/dvdy.1183

✦ Synopsis

Abstract

Annexin (Anx) 1, 2, 5, and 6 expressions were determined at the transcriptional and translational levels in the rat hepatocytes from gestational day 15 to postnatal day 17. Dramatic shifts were observed in Anx 1 and 2 levels, which peaked at day 1 and gestational day 20, respectively, and reached low levels thereafter. However, Anx 5 and 6 rates were more constant. Prenatal administration of dexamethasone (dex) resulted in a decrease of Anx 1 mRNA levels, and a strong increase in Anx 2 mRNA contents. In adult hepatocytes cultured in the presence of EGF or HGF, Anx 1 and 2 expressions resumed. By immunohistochemistry, Anx 1 was detected only in the cytoplasm of hepatocytes of 1‐ to 3‐day‐old rats, Anx 2 and 6 both exhibited a redistribution from the cytoplasm toward the plasma membrane, and Anx 5 was present in the nucleus, cytoplasm, and plasma membrane. Thus, Anx 1, 2, 5, and 6 have individual modes of expression and localization in the differentiating hepatocytes, where they might play unique roles at well defined phases of liver ontogeny. © 2001 Wiley‐Liss, Inc.