Anin vitroinvasion model for human renal cell carcinoma cell lines mimicking their metastatic abilities

✍ Scribed by Yoshifumi Nakayama; Seiji Naito; Masahiro Ryuto; Yasuaki Hata; Mayumi Ono; Katsuo Sueishi; Sohtaro Komiyama; Hideaki Roh; Michihiko Kuwano

• Book ID: 104633371
• Publisher: Springer
• Year: 1996
• Tongue: English
• Weight: 970 KB
• Volume: 14
• Category: Article
• ISSN: 0262-0898
• DOI: 10.1007/bf00128963

✦ Synopsis

We developed a modified in vitro invasion assay system using monolayers of vascular endothelial cells. A type I collagen gel was formed in plastic dishes, and overlaid with type IV collagen. Calf pulmonary arterial endothelial (CPAE) cells were seeded onto these plates, and incubated until they reached confluence. Five human renal cell carcinoma cell lines with various metastatic potentials in vivo were then seeded on the monolayer CPAE cells, and their colony formation and invasion activities were examined for 9 days. At day 4, the highly metastatic cell lines increased the number of colony foci on monolayer CPAE cells several fold higher than their poorly metastatic counterpart. The horizontal spreading patterns were also different between poorly and highly metastatic cell lines. On day 9, the number of carcinoma foci that penetrated the monolayer of CPAE cells and type IV collagen sheets into type I collagen gels in highly metastatic cell lines greatly increased as compared with that of poorly metastatic cell lines. Our in vitro invasion assay using monolayer CPAE cells would be useful to evaluate protease activities and colony formation during invasion.