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Anin silicoplatform for the design of heterologous pathways in nonnative metabolite production

โœ Scribed by Sunisa Chatsurachai, Chikara Furusawa, Hiroshi Shimizu


Book ID
119894317
Publisher
BioMed Central
Year
2012
Tongue
English
Weight
438 KB
Volume
13
Category
Article
ISSN
1471-2105

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We describe a simple method using 13 C labeling and NMR spectroscopy to determine the flux contributions of alternative pathways in Saccharomyces cerevisiae that produce the same metabolite with identical labeling patterns. Cells were incubated with a 13 C-labeled precursor for one of the branches,