Basic Cell Culture / Jeffrey W. Pollard -- Establishment, Maintenance, And Cloning Of Human Primary Cell Strains / Gareth E. Jones -- Aging Of Cultured Human Skin Fibroblasts / Calvin B. Harley -- Separation And Maintenance Of Primary T And B Lymphocytes / Derek Kinchington, Eleanor Berrie -- Establishment Of Lymphoblastoid Cell Lines / A. Doyle -- Scale-up Of Suspension And Anchorage-dependent Animal Cells / J. Bryan Griffiths -- Mycoplasma Detection / J.m. Mowles -- Short-term Chorionic Villi And Amniotie Fluid Cultures / M.l. Murer-orlando, V.m. Mcguire -- Keratinocyte Culture / Yvonne Barlow, Richard J. Pye -- Establishment Of Mouse Epithelial Lung Cell Strains And Cell Lines / Garry J. Smith -- Culture Of Human Brain Tumors On An Extracellular Matrix Derived From Bovine Corneal Endothelial Cells And Cultured Human Glioma Cells / Manfred Westphal, Marianne Hänsel, Hildegard Nausch, Eva Rohde, Hans-dietrich Herrmann --^ Embryonic Rodent Brain Cells In Culture / Narayan R. Bhat -- Human Thyroid Epithelial Cells / D.w. Williams, David Wynford-thomas -- Preparation Of Isolated Rat Liver Hepatocytes / Bjørn Quistorff, John Dich, Niels Grunnet -- Primary Cultures Of Rat Hepatocytes / John Dich, Niels Grunnet -- Preparation Of Isolated Periportal Or Perivenous Hepatocytes From Rat Liver / Bjørn Quistorff -- Primary Kidney Cells / Mary Taub -- Adipocytes / Shinobu Gamou, Yoshiko Shimizu, Nobuyoshi Shimizu -- Tissue Culture Of Skeletal Muscle / Terry A. Partridge -- Derivation And Maintenance Of Embryonic Stem Cell Cultures / Elizabeth J. Robertson -- Defining Hormone And Matrix Requirements For Differentiated Epithelia / Lola M. Reid -- Culturing Primitive Hemopoietic Cells: Long-term Mouse Marrow Cultures And The Establishment Of Factor-dependent (fdcp-mix) Hemopoietic Cell Lines / Elaine Spooncer, T. Michael Dexter --^ High Proliferative Potential Colony Forming Cells / T. Ray Bradley, George S. Hodgson, Ivan Bertoncello -- Murine Bone Marrow-derived Macrophages / E. Richard Stanley -- Long-term B Lymphoid Cultures From Murine Bone Marrow: Establishment And Cloning By Using Stromal Cell Line Ac 6.21 / Cheryl A. Whitlock, Christa E. Muller-sieburg -- Cfu-c In Agar / Vincent Praloran, Anna Bartocci -- Human Long-term Bone Marrow Culture / Armand Keating, Paul Toor -- In Vitro Clonal Culture Of Human Hematopoietic Progenitor Cells / Armand Keating, Paul Toor -- Flow Sorting For Isolating Cfu-e / Suzanne M. Watt, John M. Davis -- Production Of Human And Murine Eosinophils In Vitro And Assay For Eosinophil Differentiation Factors / Malcolm Strath, Elaine J. Clutterbuck, Colin J. Sanderson -- Clonogenic Assays For Hematopoietic And Tumor Cells Using Agar-containing Capillaries / H. Rainer Maurer, Christine Echarti -- Cytogenetic Techniques For Human Leukemias / G. John Swansbury --^ Time-lapse Cinemicroscopy / Peter N. Riddle -- Transmission And Scanning Electron Microscope Preparation Of Whole Cultured Cells / Josef Neumülller -- Double Indirect-immunofluorescent Labeling Of Cultured Cells / Christine A. Boocock -- Gene Mapping To Chromosomes By Hybridization In Situ / John R. Gosden -- In Situ Hybridization With Radiolabeled Crna Probes, Using Tissue Sections And Smears / Carolin Adams, Qutayla A. Hamid, Julia M. Polak -- Immunogold Labeling For Electron Microscopy / John Pacy -- Human Chromosome Analysis And Sorting / Judith A. Fantes, Daryll K. Green -- Flow Cytometry / Michael G. Ormerod, Patrick R. Imrie -- Fluorescence-activated Cell Sorting According To Receptor Density : Application For Isolating Transfected Cell Lines / Stella Clark -- Transfection And Transformation Of Human Thyroid Epithelial Cells / Nicholas Robert Lemoine, David Wynford-thomas --^ Expression Of Foreign Genes In Cultured Insect Cells Using A Recombinant Baculovirus Vector / Martin J. Page, Vivienne F. Murphy -- Chloramphenicol Acetyltransferase As A Reporter In Mammalian Gene Transfer / Christopher D. Corsico, Bruce H. Howard -- Immunizing Schedules For Hybridoma Production / Björn Gustafsson -- Fusion Protocol For The Production Of Mouse Hybridomas / Björn Gustafsson -- Cloning Of Hybridomas / Björn Gustafsson -- Enzyme-linked Immunosorbent Assay For Screening Of Antibodies In Hybridoma Supernatants / Björn Gustafsson -- Cryopreservation Of Hybridomas / Björn Gustafsson -- Isotype Determination Of Monoclonal Antibodies By Immunodiffusion / Björn Gustafsson -- Isoelectric Focusing And Immunofixation Of Monoclonal Antibodies / Björn Gustafsson -- Large-scale Production And Storage Of Monoclonal Antibodies And Hybridomas / Stuart A. Clark, J. Bryan Griffiths, Christopher B. Morris --^ Purification Of Murine Monoclonal Antibodies / Michael G. Baines, Andrew J. Gearing, Robin Thorpe -- Rat [cross] Rat Hybridomas / Christopher J. Dean -- Monoclonal Antibodies Against Glycosphingolipids (gsls)-gangliosides / Bruce Fletcher Clark. Edited By Jeffrey W. Pollard And John M. Walker. Includes Bibliographical References And Index.