Animal Cell Culture

✍ Scribed by Mohamed Al-Rubeai (eds.)

• Publisher: Springer International Publishing
• Year: 2015
• Tongue: English
• Leaves: 766
• Series: Cell Engineering 9
• Edition: 1
• Category: Library

✦ Synopsis

​Animal cells are the preferred “cell factories” for the production of complex molecules and antibodies for use as prophylactics, therapeutics or diagnostics. Animal cells are required for the correct post-translational processing (including glycosylation) of biopharmaceutical protein products. They are used for the production of viral vectors for gene therapy. Major targets for this therapy include cancer, HIV, arthritis, cardiovascular and CNS diseases and cystic fibrosis. Animal cells are used as in vitro substrates in pharmacological and toxicological studies. This book is designed to serve as a comprehensive review of animal cell culture, covering the current status of both research and applications. For the student or R&D scientist or new researcher the protocols are central to the performance of cell culture work, yet a broad understanding is essential for translation of laboratory findings into the industrial production. Within the broad scope of the book, each topic is reviewed authoritatively by experts in the field to produce state-of-the-art collection of current research. A major reference volume on cell culture research and how it impacts on production of biopharmaceutical proteins worldwide, the book is essential reading for everyone working in cell culture and is a recommended volume for all biotechnology libraries.

✦ Table of Contents

Front Matter....Pages i-xii
Cell Line Development....Pages 1-25
Transient Recombinant Protein Expression in Mammalian Cells....Pages 27-64
Production of Antibodies in Hybridoma and Non-hybridoma Cell Lines....Pages 65-88
Bioreactors for Mammalian Cells....Pages 89-135
Mass Transfer and Mixing Across the Scales in Animal Cell Culture....Pages 137-167
Hydrodynamic Damage to Animal Cells....Pages 169-183
Monitoring of Cell Culture....Pages 185-221
Serum and Protein Free Media....Pages 223-236
Glycosylation in Cell Culture....Pages 237-258
Modelling of Mammalian Cell Cultures....Pages 259-326
Mammalian Cell Line Selection Strategies for High-Producers....Pages 327-372
Building a Cell Culture Process with Stable Foundations: Searching for Certainty in an Uncertain World....Pages 373-406
Perfusion Processes....Pages 407-443
Single-Use Bioreactors for Animal and Human Cells....Pages 445-500
An Overview of Cell Culture Engineering for the Insect Cell-Baculovirus Expression Vector System (BEVS)....Pages 501-519
Metabolic Flux Analysis: A Powerful Tool in Animal Cell Culture....Pages 521-539
Cell Immobilization for the Production of Viral Vaccines....Pages 541-563
Cell Engineering for Therapeutic Protein Production....Pages 565-590
Proteomics in Cell Culture: From Genomics to Combined ‘Omics for Cell Line Engineering and Bioprocess Development....Pages 591-614
Metabolomics in Animal Cell Culture....Pages 615-646
Process Analytical Technology and Quality-by-Design for Animal Cell Culture....Pages 647-688
Biosafety Recommendations on the Handling of Animal Cell Cultures....Pages 689-716
Biopharmaceutical Products from Animal Cell Culture....Pages 717-757
Back Matter....Pages 759-763

✦ Subjects

Biomedicine general; Cell Biology; Stem Cells; Cell Culture