Animal Cell Biotechnology Volume 8 || Cell Bank Preparation and Characterization

✍ Scribed by Jenkins, Nigel

• Book ID: 124082130
• Publisher: Humana Press
• Year: 1999
• Tongue: English
• Weight: 169 KB
• Edition: 1
• Category: Article
• ISBN-13: 9780896035478
• DOI: 10.1385/0-89603-547-6:99

✦ Synopsis

Enabling Technologies -- Setting Up A New Cell Culture Laboratory -- Adaptation Of Mammalian Cells To Growth In Serum-free Media -- Viral Evaluation Of Animal Cell Lines Used In Biotechnology -- Molecular Methods -- Optimizing Gene Expression In Mammalian Cells -- Cytogenetic Characterization Of Recombinant Cells -- Immortalization Strategies For Mammalian Cells -- Mouse Monoclonal Antibodies: Immunization And Production Protocols -- Cell Bank Preparation And Characterization -- Dna Fingerprinting And Characterization Of Animals Cells -- Cell Evaluation Protocols -- Cell Counting And Viability Measurements -- Monitoring Animal Cell Growth And Productivity By Flow Cytometry -- Measurement Of Cell Death In Culture -- Nuclear Magnetic Resonance Methods -- Specialist Techniques -- Culturing Animal Cells In Fluidized Bed Reactors -- Gpi-anchored Fusion Proteins -- Harvesting Gpi-anchored Proteins From Cho Cells -- Hematopoietic Cells For Cellular And Gene Therapy: 1. Basic Assay Techniques -- Hematopoietic Cells For Cellular And Gene Therapy: 2. Expansion Protocols -- Cytotoxicity Testing Using Cell Lines -- Product Evaluation Protocols --measuring The Folding Dynamics Of Recombinant Proteins Secreted From Mammalian Cells -- Control Of Proteolysis In Cell Culture: Use Of Inhibitors And Engineered Cell Lines -- Monitoring Recombinant Glycoprotein Heterogeneity -- Measurement Of Cell-culture Glycosidase Activity. Edited By Nigel Jenkins. Includes Bibliographical References And Index.