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Anhydrotetracycline oxygenase activity and biosynthesis of tetracyclines in streptomyces aureofaciens

✍ Scribed by V. Béhal; Z. Hošťálek; Z. Vaněk

Book ID
105173914
Publisher
Springer Netherlands
Year
1979
Tongue
English
Weight
263 KB
Volume
1
Category
Article
ISSN
0141-5492

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Partial purification and characterizatio
Partial purification and characterization of anhydrotetracycline oxygenase of Streptomyces aureofaciens
✍ Ivana Vančurová; Miroslav Flieger; Jindřich Volc; Milan J. Beneš; Jana Novotná; 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 310 KB

Anhydrotetracycline oxygenase was purified both by affinity chromatography and by hydrophobic interaction chromatography. Molecular weight of anhydrotetracycline oxygenase was determined to be 115,000 by Sephadex G-200 gel filtration. Using preparative jsoelectric focusing the isoelectric point of t