A rapid, simple, and accurate method for the chemical assay of angiotensin-converting enzyme has been developed. The method relies on previously published method for spectrophotometric assay of angiotensinconverting enzyme activity and on the use of 2,4,6-trichloro-s-triazine (TT) as a calorimetric
Angiotensin converting enzyme: Method of assay and partial purification
โ Scribed by Frederic E. Dorer; Leonard T. Skeggs; Joseph R. Kahn; Kenneth E. Lentz; Melvin Levine
- Publisher
- Elsevier Science
- Year
- 1970
- Tongue
- English
- Weight
- 611 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0003-2697
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A relatively simple procedure is described for purifying human serum angiotensin-converting enzyme. The enzyme was purified 130,000-fold to electrophoretic homogeneity using affinity chromatography as the principal purification step. The ligand was an immobilized competitive inhibitor, D-cysteinyl-L