Angiotensin converting enzyme in cultured endothelial cells and growth medium: Relationships to enzyme from kidney and plasma
β Scribed by Shan-Fun Ching; Lyle W. Hayes; Linda L. Slakey
- Book ID
- 113124313
- Publisher
- Elsevier Science
- Year
- 1981
- Weight
- 606 KB
- Volume
- 657
- Category
- Article
- ISSN
- 0005-2744
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## Abstract Angiotensinβconverting enzyme (EC 3.4.15.1) is a carboxyterminal dipeptidyl peptidase. The enzyme catalyzes the conversion of the decapeptide angiotensin I to the octapeptide angiotensin II. In addition, the enzyme catabolizes bradykinin. Because of these actions, the enzyme is of pivot
Incubation of cultured bovine pulmonary artery endothelial cells with 200 pM of 3-isobutyl-1 -methylxanthine (IBMX) for 24 hr produced a five-to tenfold increase in cellular angiotensin converting enzyme activity (ACE) above that of untreated control cells. A lesser increase was observed in medium A