Aneuploids as a key for new molecular cloning strategies: development of DNA markers by microdissection usingTriticum aestivum-Aegilops markgrafiichromosome addition line B
✍ Scribed by Heidi Potz; Veit Schubert; Andreas Houben; Ingo Schubert; W. Eberhard Weber
- Book ID
- 104614120
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 524 KB
- Volume
- 89
- Category
- Article
- ISSN
- 0014-2336
No coin nor oath required. For personal study only.
✦ Synopsis
We established a chromosome specific DNA library of the Aegilops markgrafii chromosome B. Eight microdissected chromosomes B obtained from a monosomic T. aestivum-Aegilops markgrafii addition line were PCR-amplified and the DNA was cloned in Escherichia coli DH5a. Clones were characterized by dot blot hybridization with total Ae. markgrafii DNA. 62% of clones represented repetitive sequences and 38% low or single copy sequences. The estimated length of excised inserts varied between less than 200 bp and more than 500 bp. The average size of inserts was 310 bp.