Androgen receptors and hormone sensitivity of a human prostatic cancer cell line (PC-3) are modulated by natural beta-interferon
✍ Scribed by Sica, G. ;Dell'Acqua, G. ;Iacopino, F. ;Fattorossi, A. ;Marchetti, P. ;van der Kwast, T. H. ;Pavone-Macaluso, M.
- Book ID
- 104771288
- Publisher
- Springer
- Year
- 1994
- Tongue
- English
- Weight
- 963 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0300-5623
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✦ Synopsis
Androgen receptors are expressed at a low level in the cell line PC-3, which does not respond to either androgens or antiandrogens. If these cells are exposed to natural beta-interferon (beta-IFN) a reduction in cell growth and an increase in androgen receptors, evaluated by both biochemical and immunocytochemical techniques, occur. This increase seems not to be related to a selective block of PC-3 in any phase of the cell cycle. Pretreatment with beta-IFN determines in PC-3 cells a partial responsiveness to the androgen dihydrotestosterone as reflected by the increase in cell number. Moreover, the antiandrogen hydroxyflutamide shows agonistic properties by increasing the cell number of PC-3 cells pre-exposed to beta-IFN. When the antiandrogen is tested in combination with interferon, it produces a reduction in the beta-IFN-induced inhibition of cell growth. It is not known whether these unexpected effects are due to the increase in androgen receptors or to other mechanisms.