𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Androgen-independent growth is induced by neuropeptides in human prostate cancer cell lines

✍ Scribed by Jongsma, Johan; Oomen, Monique H.A.; Noordzij, Marinus A.; Romijn, Johannes C.; van der Kwast, Theodorus H.; Schr�der, Fritz H.; van Steenbrugge, Gert J.

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
382 KB
Volume
42
Category
Article
ISSN
0270-4137

No coin nor oath required. For personal study only.

✦ Synopsis

BACKGROUND. Androgen-independent growth leads to progressive prostate cancer after androgen-ablation therapy. This may be caused by altered specificity of the androgen receptor (AR), by ligand-independent stimulation of the AR, or by paracrine growth modulation by neuropeptides secreted by neuroendocrine (NE) cells. METHODS. We established and characterized the androgen-independent FGC-DCC from the androgen-dependent LNCaP fast growing colony (FGC) cell line. The androgenindependent DU-145, FGC-DCC, and PC-3, and the androgen-dependent LNCaP and PC-346C cell lines were used to study growth modulation of gastrin-releasing peptide (GRP), calcitonin (CT), serotonin (5-HT), and vasoactive intestinal peptide (VIP) by 3 H-thymidine incorporation. Specificity of the growth-modulating effects was tested with the anti-GRP monoclonal antibody 2A11 and induction of cAMP by neuropeptides. RESULTS. Androgen-independent growth stimulation by neuropeptides was shown in DU-145 and PC-346C. 2A11 inhibited GRP-induced 3 H-thymidine incorporation in DU-145 and PC-346C and inhibited proliferation of the FGC-DCC and PC-3 cell lines. With some exceptions, cAMP induction paralleled growth stimulation. Dideoxyadenosine (DDA) inhibited the GRP-induced growth effect in DU-145 and PC-346C, whereas oxadiazoloquinoxaline-1-one (ODQ) had no effect on 3 H-thymidine incorporation. None of the neuropeptides stimulated growth of LNCaP, FGC-DCC, or PC-3. CONCLUSIONS. GRP-induced growth of DU-145 and PC-346C was specific and cAMPmediated. Androgen-independent growth of FGC-DCC cells was mainly due to an induction of Bcl-2 expression and possibly through the activation of an autocrine and NE-like pathway, as has been shown also for the PC-3 cell line. Growth induction of non-NE cells by neuropeptides could be a possible role for NE cells in clinical prostate cancer.

📜 SIMILAR VOLUMES

Tributyrin induces differentiation, grow
Tributyrin induces differentiation, growth arrest and apoptosis in androgen-sensitive and androgen-resistant human prostate cancer cell lines
✍ Simone Maier; Ella Reich; Renate Martin; Max Bachem; Vedat Altug; Richard E. Hau 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 French ⚖ 321 KB 👁 1 views

Progression to androgen independence remains the main problem that impacts on survival and quality of life in prostate cancer patients. We have investigated the potency of tributyrin, an orally available prodrug of butyrate, to induce growth arrest, differentiation and apoptosis in LNCaP, PC-3 and T

Caveolin expression is decreased followi
Caveolin expression is decreased following androgen deprivation in human prostate cancer cell lines
✍ Pflug, Beth R.; Reiter, Robert E.; Nelson, Joel B. 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 182 KB 👁 2 views

BACKGROUND. Increased expression of caveolin has been associated with prostate cancer progression. In a mouse reconstitution model of prostate cancer, expression of caveolin was inversely related to androgen sensitivity: androgen independent clones had high caveolin expression; low caveolin expressi

Role of antioxidant systems in human and
Role of antioxidant systems in human androgen-independent prostate cancer cells
✍ Suzuki, Yasutomo; Kondo, Yukihiro; Himeno, Seiichiro; Nemoto, Kaoru; Akimoto, Ma 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 228 KB 👁 1 views

## BACKGROUND. Most prostate cancer cells respond to initial hormonal therapy; however, some of them eventually acquire resistance to the hormonal therapy. Hormone-independent prostate cancer usually exhibits resistance to chemotherapy and radiotherapy. Antioxidant systems are known to be involved

Growth-inhibitory activity of melatonin
Growth-inhibitory activity of melatonin on human androgen-independent DU 145 prostate cancer cells
✍ M. Montagnani Marelli; P. Limonta; R. Maggi; M. Motta; R.M. Moretti 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 243 KB

## Background: The pineal hormone melatonin has been shown to exert a direct oncostatic activity on neoplastic cells, particularly from breast cancer. in the present study, we evaluated the effects of melatonin on the proliferation and on the cell cycle distribution of human androgen-independent du

Epidermal growth factor induces cyclin D
Epidermal growth factor induces cyclin D1 in a human prostate cancer cell line
✍ Perry, Jaime E.; Grossmann, Michael E.; Tindall, Donald J. 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 350 KB 👁 1 views

## BACKGROUND. The human prostate carcinoma cell line, LNCaP, proliferates under stimulation by a limited number of mitogenic signals, which include members of the growth factor and steroid hormone families. Androgens and epidermal growth factor (EGF) are among the LNCaP cell mitogens. We tested t