Androgen-independent basal cell re-epithelialization, c-erbB-2 mRNA expression and androgen-dependent EGFr mRNA expression in benign prostatic hyperplasia explant cultures treated with finasteride
✍ Scribed by Simó Schwartz Jr.; Carme Caceres; Inés De Torres; Joan Morote; José M. Rodriguez-Vallejo; Jorge Gonzalez; Jaume Reventos
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- French
- Weight
- 125 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
We have analyzed the effects of the 5␣-reductase inhibitor, finasteride (MK906), on the mRNA expression of the epidermal growth factor receptor and c-erbB-2 genes, in benign prostatic hyperplasia explant cultures treated with testosterone and with testosterone plus finasteride. A decrease of the epithelial cell content and an androgen-independent basal cell re-epithelialization was observed during the first 10 days of culture, suggesting a role of basal cells as stem cells involved in androgen-independent epithelial regeneration. Using a semi-quantitative reverse transcription polymerase chain reaction technique, we observed a significant decrease in expression of the epidermal growth factor receptor in the cultures treated with finasteride whereas no effect of finasteride on c-erbB-2 transcription was detected, although the expression of both genes was increased by dihydrotestosterone.