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Anchorage dependent changes in transport of glucose, adenosine, uridine and leucine in 3T3 cells

✍ Scribed by Haruki Otsuka; Merwin Moskowitz

Book ID
102883605
Publisher
John Wiley and Sons
Year
1975
Tongue
English
Weight
659 KB
Volume
86
Category
Article
ISSN
0021-9541

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✦ Synopsis

There is a low uptake of leucine in suspension cultures of 3T3 cells relative to the uptake in sparse monolayer cultures. The pattern of uptake of deoxyglucose is similar in suspension and monolayer cultures and changing the medium or adding serum stimulates uptake under both culture conditions.

The uptake of uridine and adenosine is greater in suspension culture than in monolayer culture. Cells do not multiply in suspension culture but do multiply in monolayer culture and thus there is a correlation between uptake of leucine and conditions which stimulate cell multiplication, but no correlation of the uptake of deoxyglucose, uridine and adenosine with these conditions.

πŸ“œ SIMILAR VOLUMES

Difference in transport of leucine in at
Difference in transport of leucine in attached and suspended 3T3 cells
✍ Haruki Otsuka; Merwin Moskowitz πŸ“‚ Article πŸ“… 1975 πŸ› John Wiley and Sons 🌐 English βš– 702 KB

## Abstract 3T3 cells in subconfluent culture take up leucine through a transport system which has a relatively high affinity for leucine (M system). When the culture becomes confluent, the M system is turned off and leucine is transported by another system which has a low affinity for leucine (S s

Transport of amino acids in intact 3T3 a
Transport of amino acids in intact 3T3 and SV3T3 cells. Binding activity for leucine in membrane preparations of ehrlich ascites tumor cells
✍ Cecchini, Gary ;Lee, Mary ;Oxender, Dale L. πŸ“‚ Article πŸ“… 1976 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 315 KB

## Abstract Transport of amino acids into 3T3 and SV3T3 (SV40 virus‐transformed 3T3) cells was measured on glass cover slips. The 3T3 and SV3T3 cells contain both A (alanine preferring) and L (leucine preferring) systems for neutral amino acid transport. Initial rates of uptake of amino acids are a