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Anaplerotic roles of pyruvate carboxylase in mammalian tissues

โœ Scribed by S. Jitrapakdee; A. Vidal-Puig; J. C. Wallace


Publisher
Springer
Year
2006
Tongue
English
Weight
360 KB
Volume
63
Category
Article
ISSN
1420-682X

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Optical assay of pyruvate carboxylase in
โœ J. Berndt; B. Messner; T. Turkki; E. Weissmann ๐Ÿ“‚ Article ๐Ÿ“… 1978 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 262 KB

A technique to measure the activity of pyruvate carboxylase spectrophotometritally in crude liver homogenates is described. The assay is based on the transformation of oxaloacetate, which is formed during the carboxylation reaction, into citrate in the presence ofexcess acetyl CoA and citrate syntha