Analyzing the structure of polypeptides in membranes by fluorescence quenching
โ Scribed by London, E.
- Book ID
- 119410307
- Publisher
- Biophysical Society
- Year
- 1994
- Tongue
- English
- Weight
- 159 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0006-3495
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๐ SIMILAR VOLUMES
Fluorescence quenching is the loss of fluorescence intensity which is observed when a fluorescent molecule or group interacts with another molecule or group, called the quencher. By use of tryptophan residues of proteins, together with specific probe molecules, quenching can be applied to problems o
The transverse organisation of ubiquinone in mitochondrial membranes was investigated by quenching a set of fluorescent fatty acids. We show that the fluorescent moiety of the probes is located at a graded series of depths in the mitochondrial membrane. The probes sense the characteristics of the li