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Analyzing single protein molecules using optical methods

✍ Scribed by Petra Schwille; Ulrich Kettling


Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
112 KB
Volume
12
Category
Article
ISSN
0958-1669

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✦ Synopsis


Studies on single protein molecules have advanced from mere proofs of principle to insightful investigations of otherwise inaccessible biological phenomena. Recent studies predict a tremendous number of possible future applications. The longterm vision of biologists to watch single molecular processes in real time by peering into a cell with three-dimensional resolution might finally be realized. Another fascinating perspective is the identification and selection of single favorable variants from complex libraries of diverse biomolecules.


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Single copies of four different phenolate ion mutants of the green fluorescent protein (GFP) exhibit a complex blinking and fluctuating behavior, a phenomenon that is hidden in measurements on large ensembles. Both total internal reflection microscopy and scanning confocal microscopy can be used to