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Analyzing real-time PCR data by the comparative CT method

✍ Scribed by Schmittgen, Thomas D; Livak, Kenneth J


Book ID
109946634
Publisher
Nature Publishing Group
Year
2008
Tongue
English
Weight
471 KB
Volume
3
Category
Article
ISSN
1750-2799

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## Abstract The polymerase chain reaction (PCR) is widely used to ascertain absolute or relative changes in the expression levels of specific genes as a function of cell type or in response to changes in environmental stimuli. Real‐time PCR is an advance which allows for the analysis of gene expres