Analytical Utilization of Phenylfluorone as a Hydrogen Donor Substrate for Peroxidase

✍ Scribed by Zhong-Xian Guo; Han-Xi Shen; Li Li

• Book ID: 102614417
• Publisher: Elsevier Science
• Year: 1999
• Tongue: English
• Weight: 63 KB
• Volume: 61
• Category: Article
• ISSN: 0026-265X
• DOI: 10.1006/mchj.1998.1679

✦ Synopsis

Phenylfluorone (PF) is used as an efficient hydrogen donor substrate for peroxidase. Based on the horseradish peroxidase (HRP)-hydrogen peroxide-PF catalytic system, the spectrophotometric methods for the measurement of HRP activity and the determination of hydrogen peroxide are proposed. The quantitative assay of HRP is sensitive to 9.2 ϫ 10 Ϫ12 mol/liter, and the calibration curve for hydrogen peroxide by a equilibrium procedure is linear in the range from 1.2 ϫ 10 Ϫ7 to 1.6 ϫ 10 Ϫ5 mol/liter. The system can be easily coupled with a glucose oxidase-catalyzed reaction, and glucose in human serum has been determined with the same results by a phenol-4-aminoantipyine method. Phenylfluorone offers advantages over some presently used chromogenic substrates in the sense of sensitivity and simplicity of the substrate system.