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Analytical separation of Trichoderma Reesei cellulases by Capillary Zone Electrophoresis

✍ Scribed by Maha Y. Khaled; Harold McNair; Lee Polite; Richard Pauls

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
364 KB
Volume
7
Category
Article
ISSN
1040-7685

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✦ Synopsis

Abstract

The Capillary Zone Electrophoretic separation of the four common enzymes of Cellulase, the endo‐(EG I, EG II) and the exo‐(CBH I, and CBH II) glucohydrolases from the fungi Trichoderma Reesei, is presented. The separation using underivatized fused silica capillaries with 0.03 M Na~2~HPO~4~ at an optimum pH of 9.2 proved successful, rapid, and reproducible. Variations in buffer type, ionic strength, pH, temperature, and reduced electroosmotic flow and their effects on the separation were investigated revealing a significant effect on the efficiency, the separation, and the integrity of the enzymes. Β© 1995 John Wiley & Sons, Inc.

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