The book aims to describe the microscopic characterization of the soft matter in the light of new advances acquired in the science of microscopy techniques like AFM; SEM; TEM etc. It does not focus on the traditional information on the microscopy methods as well as systems already present in different books, but intends to answer more fundamental questions associated with commercially important systems by using new advances in microscopy. Such questions are generally not answered by other techniques. The contents of the book also reflect this as the chapters are not based on describing only material systems, but are based on the answering the problems or questions arising in their characterization. Both qualitative as well as quantitative analysis using such microscopic techniques is discussed. Moreover, efforts have been made to provide a broader reach as discussions on both polymers as well as biological matter have been included as different sections. Such a text with comprehensive overview of the various characterization possibilities using microscopy methods can serve as a valuable reference for microscopy experts as well as non-experts alike