Analytical capillary isotachophoresis of human serum lipoproteins
✍ Scribed by Prof. Dr. med. Gerd Schmitz; Christoph Möllers; Volker Richter
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 700 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
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Human serum was incubated with representative portions of polyvinyl chloride (PVC) blood storage bags and vascular prostheses. The in vitro interaction process with lipoprotein was followed by polyacrylamide gel electrophoresis (PAGE) using sudan black and nitroblue tetrazolium (NBT) in the prestain
Combining specific enzymatic derivatization of cholesterol or triglycerides with capillary isotachophoresis (CITP), human serum lipoproteins are separated into 14 lipoprotein subfractions, monitored and quantitated by direct capillary UV detection. By comparing the separation patterns of human serum