โ Scribed by Hassan Y. Aboul-Enein
No coin nor oath required. For personal study only.
Reports up-to-date research developments on purifying and isolation large organic molecules. The text provides information on high-performance liquid chromatography and capillary electrophoresis (CE) as tools for analyzing biomacromolecules and developing new biochemical and medicinal compounds. It applies biochemical separation technology to the study of macromolecules such as proteins, polysaccharides, nucleic acids and more.
Preface......Page 6
Contents......Page 8
Contributors......Page 12
Analysis of HbA1c and Some Hb
Variants by H PLC......Page 15
Analysis of Posttranslational Modifications in Recombinant Proteins by HPLC and Mass Spectrometry......Page 27
Analytical and Preparative Separations of Peptides by Capillary and Free-Flow Zone Electrophoresis......Page 53
Bioaffinity Chromatography......Page 113
Characterization and Partial Purification of Steroidogenic Factors from Thymic Epithelial Cell-Conditioned Medium......Page 181
Determination of Affinity Constants of Lectins for Sugars by Affinity Capillary Electrophoresis......Page 201
Displacement Chromatography of Biomolecules......Page 217
High-Performance Membrane Chromatography of Proteins......Page 269
HPLC Purification of Recombinant Proteins......Page 315
Isolation, Purification, and Characterization of Human Seminal Plasma Proteins and Their immunological Behavior In Vitro......Page 345
Isolation, Purification, and Structural Study of Allergenic Proteins......Page 367
Multiangle Combined Light Scattering with HPLC with Examples for Biopolymers......Page 383
Purification and Characterization of Connective Tissue Growth Factor Using Heparin Affinity Chromatography......Page 411
Slalom Chromatography......Page 429
Simultaneous Chromatographic Separation of Ceruloplasmin and Serum Amine Oxidase......Page 445
Fast, Single-Step Affinity Chromatography Purification of Hemoglobin......Page 459
Index......Page 471
๐ SIMILAR VOLUMES
<span>Cell separation, which was once limited to merely being a basic technique for fractionating different cell populations, has come a long way in the last two decades. New, advanced and more speci?c and selective techniques have emerged as the demand for isolating a speci?c cell type for various
Hardbound. The new technologies in drug research such as combinatorial chemistry, high throughput screening, and robotics have made it possible to synthesise millions of molecules for thousands of screens. This volume presents the theory and applications of a wide range of separation methods used in
This book provides key information about the instrumental analytical methods which are the most used in quantitative analysis. A theoretical knowledge of each method is discussed. The methods are illustrated with several examples covering a wide range such as pharmacy, biochemical, environmental and
<p><span>This book provides key information about the instrumental analytical methods which are the most used in quantitative analysis. A theoretical knowledge of each method is discussed. The methods are illustrated with several examples covering a wide range such as pharmacy, biochemical, environm
Describes the procedures for collection of samples, sample preparation, and analysis of CWC-related chemicals. It deals with analytical procedures that can be followed in well-equipped off-site laboratories (designated laboratories), as well as the on-site analytical procedures that the OPCW inspect