## Identification of different galectins by immunoblotting after two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients Vertebrate soluble B-galactoside-binding lectins form a growing protein family that recently have been named galectins. Seven different galectins have b
Analysis ofTheileria parvaimmunodominant schizont surface antigen by two-dimensional polyacrylamide gel electrophoresis and immunoblotting
β Scribed by C. Sugimoto; L. M. Mutharia; W. C. Brown; T. W. Pearson; T. T. Dolan; P. A. Conrad
- Publisher
- Springer-Verlag
- Year
- 1992
- Tongue
- English
- Weight
- 976 KB
- Volume
- 78
- Category
- Article
- ISSN
- 1432-1955
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract This paper describes an improved scheme for the identification of antigens in crude extracts recognized by specific antibodies when analyzed by a combination of twoβdimensional gel electrophoresis and immunoblotting. First, protein components in gels are electrophoretically transferred
Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) followed by mass spectrometric identification of the proteins in the protein spots has become a central tool in proteomics. CMT167(H), CMT64(M) and CMT170(L) cell lines, selected from a spontaneous mouse lung adenocarcinoma, with high-, m
Phenolic compounds present in conifer tissues interfere with the analysis of conifer proteins by twodimensional (2-D) gel electrophoresis, causing gel streaking and distortion of protein patterns. A protocol was established by modifying the Hochstrasser method and using the Millipore 2 D Investigato