Analysis of yohimbine alkaloid from Pausinystalia yohimbe by non-aqueous capillary electrophoresis and gas chromatography-mass spectrometry

✍ Scribed by Qinhua Chen; Peng Li; Zhuo Zhang; Kaijun Li; Jia Liu; Qiang Li

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 792 KB
• Volume: 31
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200800055

✦ Synopsis

Abstract

In the present work, the qualitative and quantitative analysis of Pausinystalia yohimbe‐type alkaloids in the barks of Rubiaceae species is presented using different analytical approaches. Extracts of P. yohimbe were first examined by GC‐MS and the major alkaloids were identified. The quantitation of yohimbine was then accomplished by non‐aqueous CE (NACE) with diode array detection. This approach was selected in order to use a running buffer fully compatible with samples in organic solvent. In particular, a mixture of methanol containing ammonium acetate (20 mM) and glacial acetic acid was used as a BGE. The same analytical sample was subjected to GC‐MS and NACE analysis; the different selectivity displayed by these techniques allowed different separation profiles that can be useful in phytochemical characterization of the extracts. The linear calibration ranges were all 10–1000 μg/mL for yohimbine by GC‐MS and NACE analysis. The recovery of yohimbine was 91.2–94.0% with RSD 1.4–4.3%. The LOD for yohimbine were 0.6 μg/mL by GC‐MS and 1.0 μg/mL by NACE, respectively. The GC‐MS and NACE methods were successfully validated and applied to the quantitation of yohimbine.