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Analysis of Tween 80 as an esterase/ lipase substrate for lipolytic activity assay

โœ Scribed by Francisco J. Plou; Manuel Ferrer; Oscar M. Nuero; Maria V. Calvo; Miguel Alcalde; Fuensanta Reyes; Antonio Ballesteros


Book ID
110268004
Publisher
Springer-Verlag
Year
1998
Tongue
English
Weight
168 KB
Volume
12
Category
Article
ISSN
0951-208X

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A new type of fluorogenic alkyldiacyl glycerols was synthesized and used as fluorogenic substrates for the analysis of lipase activities and stereoselectivities. These compounds contain perylene as a fluorophore and the trinitrophenylamino ("P) residue as a quencher. Both substituents are covalently