Analysis of tramadol in pharmaceutical preparations by high performance thin layer chromatography

✍ Scribed by S. N. Meyyanathan; Pradeep Kumar; B. Suresh

• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 267 KB
• Volume: 26
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200301541

✦ Synopsis

Abstract

A new simple, precise, rapid, and selective high‐performance thin layer chromatography (HPTLC) method has been developed for the analysis of tramadol in pharmaceutical formulations. The method uses chlorzoxazone as an internal standard. The stationary phase was silica gel 60F~254~ prewashed with methanol; ethyl acetate‐methanol‐ammonia solution 7 + 1 + 0.5 (v/v) was used as mobile phase. Detection and quantification were performed densitometrically at λ = 275 nm. The linear range of the analysis was 1.0–2.5 μg and the percentage recovery was 104.6%.