Analysis of the sheep MHC using HLA class I, II, and C4 cDNA probes

✍ Scribed by Patrick Chardon; Marek Kirszenbaum; Philippa R. Cullen; Claudine Geffrotin; Charles Auffray; Jack L. Strominger; Daniel Cohen; Marcel Vaiman

• Publisher: Springer-Verlag
• Year: 1985
• Tongue: English
• Weight: 620 KB
• Volume: 22
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00430918

✦ Synopsis

Four cDNA probes for the human major histocompatibility complex (MHC) were used to investigate the sheep MHC, in conjunction with serological typing for ovine lymphocyte antigen (OLA). Lymphocytes from a family (two parents and five offspring) of Romanov sheep were subjected to genomic DNA digestion by the restriction endonuclease Eco RI, followed by gel electrophoresis. A single Southern blot representing all seven individuals was then consecutively hybridized with the class I, alpha-DC, beta-DR, and C4 probes, which were originally designed to identify HLA class I, class II (DC and DR), and C4 products, respectively. Using each of the three class I/class II probes, several bands showing DNA polymorphism were detected. The segregation of these bands in the five offspring exactly paralleled the OLA haplotype segregation established by serological typing. A further eight individuals carrying haplotypes which were phenotypically identical to those in the above-mentioned family showed bands in the corresponding positions when tested with the same three probes. Using the C4 probe, no polymorphism was detected in these fifteen individuals.