Analysis of the multiple transcripts of the Ddras gene duringDictyostezium discoideum development

✍ Scribed by Reymond, Christophe D. ;Thompson, Nancy A.

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 720 KB
• Volume: 12
• Category: Article
• ISSN: 0192-253X
• DOI: 10.1002/dvg.1020120122

✦ Synopsis

Transcripts from the Dd ras gene can only be detected once starved cells have begun to aggregate (Reymond ef a/., Cell 39: 14 1984). We show in this report that the three transcripts which originate from Dd ras during normal development differ in their 5' ends. In suspension of starved single cells, one major Dd ras RNA accumulates upon addition of CAMP. It seems that the CAMP regulation of Dd ras expression happens both at the transcriptional and post-transcriptional level. An RNA secondary structure present in the 5' untranslated region of the gene is proposed to be important in this post-transcriptional regulation.

of the cells was impaired [Van Haastert et-al., 19871, that the inositol triphosphate and hexakisphosphates were elevated [Europe-Finner et al., 19881, and that some receptor properties were modified through a pathway which may involve protein kinase C activation [Luderus et al., 19881. We have now mapped the three transcription start sites from the Dd rus gene. Furthermore, we can show that the gene is regulated both a t the transcriptional and post-transcriptional level upon external addition of CAMP to Dictyostelium cells. An RNA secondary structure is proposed to play a role in the latter regulation.