✦ LIBER ✦
Analysis of the major mercapturic acid pathway metabolites of benzo[a]pyrene found in rat urine by nano-electrospray mass spectrometry
✍ Scribed by Yang Yang; Joseph Rafter; Jan-Åke Gustafsson; Jan Sjövall; William J. Griffiths
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 165 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0951-4198
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✦ Synopsis
Nano-electrospray has been used in combination with high resolution and tandem mass spectrometry in the analysis of the major mercapturic acid pathway metabolites of benzo[a]pyrene (BP). Accurate mass measurements indicate that the [MÀH]
À ion of the major metabolite has a chemical formula C 25 H 22 NO 6 S, which corresponds to the deprotonated form of tetrahydro-trihydroxy-BP-S-N-acetylcysteine. Tandem mass spectrometry of this [MÀH] À ion results in a collision induced dissociation spectrum identical to that of synthetic 7,8,9,10-tetrahydro-8,9,10-trihydroxy-BP-7-S-N-acetylcysteine.