𝔖 Bobbio Scriptorium
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Analysis of the kinetic mechanism of halophilic NADP-dependent glutamate dehydrogenase

✍ Scribed by María José Bonete; Mónica L. Camacho; Eduardo Cadenas


Book ID
113268080
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
519 KB
Volume
1041
Category
Article
ISSN
0167-4838

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Purification and properties of the NADP-
✍ F. Pamula; J. F. Wheldrake 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 523 KB

NADP-dependent glutamate dehydrogenase from Dictyostelium discoideum was purified 9300 fold with a yield of 4.6%. The enzyme is a hexamer of apparent molecular weight 294 kDa on Sephacryl S400 and a subunit molecular weight of 52 kDa as determined by SDS gel electrophoresis. The apparent Kms for alp