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Analysis of the induction and deinduction of tyrosine aminotransferase in enucleated HTC cells

✍ Scribed by R. D. Ivarie; W. J.-W. Fan; G. M. Tomkins

Publisher
John Wiley and Sons
Year
1975
Tongue
English
Weight
651 KB
Volume
85
Category
Article
ISSN
0021-9541

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✦ Synopsis

Anucleate HTC cells have been used to determine the importance of the nucleus in the regulation of the intracellular levels of tyrosine aminotransferase (TAT) in hepatoma tissue culture (HTC) cells. In the absence of the nucleus, neither the induction of the enzyme by dexamethasone nor its deinduction upon removal of the hormone occurs. Degradation of the enzyme takes place when protein synthesis is inhibited in anucleates by cycloheximide. Therefore, the maintenance of induced levels of enzyme activity after dexamethasone withdrawal from pre-induced anucleates suggests that the nucleus is required for the inactivation of the TAT mRNA.

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