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Analysis of the expression of biomarkers in urinary bladder cancer using a tissue microarray

✍ Scribed by Loleta D. Harris; Jorge De La Cerda; Tomasz Tuziak; Daniel Rosen; Lianchun Xiao; Yu Shen; Anita L. Sabichi; Bogdan Czerniak; H. Barton Grossman

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
432 KB
Volume
47
Category
Article
ISSN
0899-1987

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✦ Synopsis

Abstract

Dysregulation of Akt, PTEN, Drg‐1, Cx‐26, and L‐plastin expression appear to be important in the progression of various cancers. Their expression in bladder cancer has not been well characterized. To assess the expression of these genes and their relationship to the outcome of bladder cancer, we used a bladder cancer tissue microarray (TMA) of 251 transitional cell carcinomas. We quantitated immunohistochemical staining of each protein using both automated and manual methods and correlated the expression levels with the clinicopathologic characteristics of the tumor and patient survival. Overall, the results from both automated and manual analyses were similar. We found a significant correlation between the expression of PTEN, Cx‐26 and L‐plastin with known clinically important pathologic features of bladder cancer (tumor grade, stage, and growth pattern). Aberrant localization patterns of Cx‐26 and Drg‐1 were observed in bladder tumors. There was also a significant correlation in expression among pAkt, PTEN, and L‐plastin. Although the expression of these genes correlated with factors known to be associated with patient outcome, none of them was an independent predictor of progression‐free or overall survival. © 2008 Wiley‐Liss, Inc.

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